In order to obtain good breeding results a number of environmental factors need to acheive a minimal level. Temperature, humidity, feed, enrichment, sound levels, daylight hours and intesity and ventilation as well as handling by staff and scientist are examples of factoras that influence the outcome.
Mice should always be bred with a single male in the cage. Depending on cage size and institutional norms one or more females can be bred to the stud male simultaneously. Females of the most common inbred strains remain productive for about six months. It is important to set aside breeders for the next generation.
When all external factors have been established it is important to run the breeding colony to make sure the animals are doing fine and produce effectively. It is all too common to be overly optimistic when planning preparation time for the experiments. Include a safety margin both on time to completion and number of animals in each litter. It is important to make sure that results will be statistically significant and at the same time consider the 3R and not produce surplus animals.
kGen can plan your breeding to produce a weekly number of animals satisfying requirements om genetic setup, sex, age etc.
It is also possible to plan for a fixed number of animals at a given timepoint.
*Plan breeding for single or repeating experiments
*Optimize complex breeding schemes
*Trouble shoot problem breedings
*Speed congenic breeding
*Set up breeding schemes
*Implement colony management software
*Maintain breeding colony through software systems
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